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Fig. 2 | Fluids and Barriers of the CNS

Fig. 2

From: Blue light exposure collapses the inner blood-retinal barrier by accelerating endothelial CLDN5 degradation through the disturbance of GNAZ and the activation of ADAM17

Fig. 2

Blue light induces ADAM17 phosphorylation and activates ADAM17 in treated endothelial cells. After blue light stimulation, the phosphorylation level of ADAM17 on T735 was detected by immunoblotting. Representative images and histograms showed a remarkable phosphorylation of ADAM17 in A illuminance-dependent and B time-dependent manner. (**p < 0.01, ***p < 0.001, indicates statistical difference from the control group). C, D ADAM17 kinetics assay was performed by fluorometric method in accordance with the manufacture’s protocol. The kinetic curve of resting endothelial cells was flattened with incubation time, whereas the steep slope of group with blue light (160 lx, 1 h) stimulation represents the rapid activation of ADAM17 by blue light. C bEnd.3 cell. D HREC

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Fluids and Barriers of the CNS

ISSN: 2045-8118