Table 2 Immunohistochemical visualization of dural lymphatic structures depending on fixation method
Lymphatic structures | Fixation method | ||
|---|---|---|---|
Method #1 (PFA 4.0%) | Method #2 (PFA 0.5%) | Method #3 (Freeze) | |
Absence of lymphatic structures (n; %) | 7/7 (100%) | 2/6 (33%) | 1/17 (6%) |
Presence of lymphatic structures (n; %) | 0/ (0%) | 4/6 (67%) | 16/17 (94%) |
Lymphatic vessels with blood vessels (n; %) | - | 1/6 (17%) | 10/17 (59%) |
Lymphatic vessels without blood vessels (n; %) | - | 2/6 (33%) | 10/17 (59%) |
Clusters of LYVE-1-expressing cells (n; %) | - | 1/6 (17%) | 7/17 (41%) |