Fig. 8

Aged choroid plexus epithelial cells display reduced mitochondrial membrane potential and increased mitochondrial ROS production. A Colocalization of MitoTracker Green and TMRM in choroid plexus explants (left). Schematic of MitoSOX or TMRM incubation and 2-PM imaging (ACSF: artificial cerebrospinal fluid). B MitoSOX representative 2-PM images of 3–4 and 22–24 months old (m.o.) choroid plexus epithelial cells. C Quantification of MitoSOX fluorescence mean intensity at both ages. n = 3 animals per age. D TMRM representative 2-PM images of 3–4 and 22–24 m.o. choroid plexus epithelial cells. E Quantification of TMRM fluorescence mean intensity at both ages. F Schematic of co-incubation of TMRM and rotenone or oligomycin A (OLIG. A). G TMRM representative 2-PM images of 3–4 and 22–24 m.o. treated choroid plexus epithelial cells. H Quantification of TMRM fluorescence mean intensity after treatment with rotenone (left) or OLIG. A (right). n = 3–4 animals per age. Data are mean ± s.e.m. Un-paired t-test, *p < 0.05. A.U., arbitrary unit. Scale bars: 5 µm (A), 10 µm (B, D, G)