Fig. 1

Analysis of cytokine gene expression, cell growth, effective cytokine concentration and cell density. a IL-6 and IL-10 gene expression levels measured with qPCR in isolated brain microvessels (MV), cortex (CX) and hippocampus (HP) of wild type (WT) and apolipoprotein B-100 (APOB-100) transgenic mice. IL-10 levels are under the detection limit. b Cell viability assay on primary brain microvascular endothelial cells isolated from WT and APOB-100 transgenic mice. c Concentration dependent effects of IL-6, IL-10 and IL-6 + IL-10 on WT endothelial cell viability. d Density of primary microvascular endothelial cells isolated from WT and APOB-100 transgenic mice following IL-6, IL-10 and IL-6 + IL-10 cytokine treatments at 50 ng/ml concentration. #: significant change between WT and APOB-100 cells (p < 0.05); *: significant change due to cytokine treatment compared to the control group with the same genotype (p < 0.05)