Fig. 6

Genomic location of DMRs in “aged” poststroke BBB. Volcano plots of old post-stroke DMRs within A gene promoters B non-promoter regions. The x-axis represents percent methylation difference from age-matched controls and the y-axis reflects − log10(q-value). Significant DMRs are depicted in red (methylation differences > 25% and q-value < 0.01), and nonsignificant DMRs are in black. Violin plots of DMRs located within genes involved in C cell junction and the actin cytoskeleton, D angiogenesis and endothelial genes, E signaling pathways and transcription factors, F transporters, receptors, and channels, G extracellular matrix, H endothelial to mesenchymal transition, (I) inflammation, J mitochondria. All DMRs, regardless of significance, are plotted for select endothelial genes. Genes containing significant DMRs are marked with an asterisk (*). The y-axis represents percent methylation difference, with DMRs colored by genomic location: promoters in red, gene body in blue, and other (1–5 kb upstream of transcription start site or no genomic location) in gray