Fig. 4

Comparison of poststroke BBB DNA methylome and transcriptome profile in young (6 month) mice. A Volcano plot of differentially expressed genes (DEGs). X-axis represents log2 fold change, and y-axis is the − log10(adjusted p-value). Significant DEGs (absolute value of log2FC > 0.58 and adjusted p-value < 0.05) are red, and DEGs with no statistical difference are black. B Gene over-representation analysis summaries for upregulated DEGs (top) and downregulated DEGs (bottom). Enriched GO terms were selected based on statistical significance (q-value < 0.01) and biological significance to endothelial cells. C Venn Diagrams demonstrating the number of DEGs with differentially methylated promoters (left) or non-promoter regions (right). Only significant DEGs and DMRs were counted. If a gene contained multiple DMRs, the gene was only counted once. D Pearson correlation between changes in gene expression and either promoter methylation (left) or non-promoter methylation (right) for genes with significant changes in gene expression and methylation, with log2FC on the x-axis and methylation changes on the y-axis. Neither promoter region methylation (R = − 0.045, p = 0.67) nor non-promoter region methylation (R = − 0.019, p = 0.52) negatively correlate with gene expression. Labeled genes are relevant to endothelial cell biology