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Fig. 5 | Fluids and Barriers of the CNS

Fig. 5

From: Blood tissue Plasminogen Activator (tPA) of liver origin contributes to neurovascular coupling involving brain endothelial N-Methyl-D-Aspartate (NMDA) receptors

Fig. 5

Endothelial tPA controls NVC. A Schematic representation of the generation of VE-Cadherin Cre/tPA Lox mice. The cadherin 5 promoter was used to drive expression of Cre recombinase in the vascular endothelium. LoxP sites were flanked in the exon 3 of tPA gene. This configuration carries out the deletion of endothelial tPA gene while conserving tPA in other cells. B Colormap corresponds to the activation map related ∆CBF changes during whisker stimulations of VECad-CreΔtPA mice and their wild type littermates. Maps were obtained by subtraction of stimulation frames and resting frames. Warm colours indicate an elevation of CBF during whisker stimulations. C Time course of % CBF increase (mean ± SEM) during whisker stimulations () of VECad-CreΔtPA () and their littermates (). D Box plots show the variations of % CBF increase from baseline during whisker stimulation of VECad-CreΔtPA mice and their littermates. Box plots with medians, 1st and 3rd quartiles, min and max with values for each mouse. *p < 0.05 from VECad-CreWT, Mann–Whitney test, n = 14/17 VECad-CreWT/ VECad-CreΔtPA

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