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Fig. 4 | Fluids and Barriers of the CNS

Fig. 4

From: Blood tissue Plasminogen Activator (tPA) of liver origin contributes to neurovascular coupling involving brain endothelial N-Methyl-D-Aspartate (NMDA) receptors

Fig. 4

Intravenous administration of bradykinin type 2 receptor agonist (B2Rag) influences NVC in a tPA-dependent manner. A Schematic representation of the experimental timeline of whisker stimulation paradigm: 3 trains of stimulations were made on laser speckle flowmetry 10 min after IV injection of B2Rag at different doses (0, 7, 15 and 30 µg/kg). Between each dose, the return of CBF to baseline was waited. B Colormap corresponds to the activation map related ∆CBF changes during whisker stimulation in tPANull mice and their wild type littermates for the different doses of B2Rag (0, 7, 15 and 30 µg/kg). Warm colours indicate an elevation of CBF during whisker stimulations. C Time course of % CBF increase (mean ± SEM) during whisker stimulations () in tPANull mice () and their littermate (), at different doses of B2Rag (0, 7, 15 and 30 µg/kg). D, E Box plots show the variations of % CBF increase from baseline during whisker stimulation in tPANull mice and their wild type littermates for the different doses of B2Rag (0, 7, 15 and 30 µg/kg). Box plots with medians, 1st and 3rd quartiles, min and max with values for each mouse. *p < 0.05 from 0 mg/kg or tPAWT, Two-Way-ANOVA and Uncorrected Fisher’s LSD tests, n = 8 tPAWT, n = 6 tPANull. F Box plots representing ELISA analyses of total tPA in tPAWT mice treated or not with the B2Rag. A saline solution or 60 µg/kg of B2Rag were injected intravenously and plasma samples were collected 5 min later and subjected to ELISA analysis. Box plots with medians, 1st and 3rd quartiles, min and max with values for each mouse. *p < 0.05 from Vehicle, Mann–Whitney test, n = 4 Vehicle, n = 5 B2Rag

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