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Fig. 3 | Fluids and Barriers of the CNS

Fig. 3

From: Long-term administration of CU06-1004 ameliorates cerebrovascular aging and BBB injury in aging mouse model

Fig. 3

CU06-1004 reverses H2O2-induced senescence in HBMECs. A Senescence-associated β-galactosidase (SA-β-Gal) staining in human brain microvascular endothelial cells (HBMECs) treated with 100 μM H2O2 for 1–2 days, with or without CU06-1004 (1004), and then maintained in fresh media for 5 days. Representative microscopic images were captured with phase-contrast microscopy. B Quantification of SA-β-Gal–positive cells shown in A. C–E The relative mRNA levels of p16 and p21 in senescent HBMECs following exposure to H2O2 or CU06-1004 were quantified by reverse transcriptase–polymerase chain reaction. GAPDH, glyceraldehyde-3-phosphate dehydrogenase. All data were analyzed with one-way analysis of variance, followed by Tukey’s multiple comparison test. ###P < 0.001 vs. control. ***P < 0.001 vs. H2O2. n.s., not significant. Results are presented as the mean, and error bars represent the standard error of the mean

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