Table 1 Primer list used in quantitative real-time PCR
Gene Name | (5') Forward Primers (3') | (5') Reverse Primers (3') |
|---|---|---|
GAPDH | GGTGGTCTCCTCTGACTTCAACGT | GTGGTCGTTGAGGGCAATG |
Sox18 | TTCCATGTCACAGCCCCCTAG | GACACGTGGGAACTCCAG |
Zic3A | CAGCGACCGTAAGAAGCACA | ATAGCGGGTGGAGTGGAAGA |
Zic3B | TGTGCATACCTCGGACAAGC | GTTATGGAGGGTGGCTGCT |
ABCB1/P-gp | TTGTTTGCCACCACGATAGC | TTCTGCCCACCACTCAACTG |
VE-cadherin | TCACGATAACACGGCCAACA | TGGCATCCCATTGTCTGAGA |
PECAM1 | GAGTATTACTGCACAGCCTTCA | AACCACTGCAATAAGTCCTTTC |
Plvap | GCAGATGTGGCTGAATGCTC | CGCAGCTCTTGTTCATGTCC |
OCLN | CAGCAGCGGTGGTAACTTTG | TCCCTGATCCAGTCCTCCTC |
TJP1/ZO-1 | TGATCATTCCAGGCACTCG | CTCTTCATCTCTACTCCGGAGACT |
CLDN5 | GTTCGTTGCGCTCTTCGTGA | GCTCGTACTTCTGCGACACG |
Mfsd2a | CCCTATGAAGCCCAGCAGTC | TAAAGTGGCCGAGAGCATGA |
SLC2A1/Glut1 | GCTATGGGGAGAGCATCCTG | AAGGCCAGCAGGTTCATCAT |
ABCC1/MRP1 | CAAGGTGGATGCGAATGAGG | TGAGGAAGTAGGGCCCAAAG |
ABCG2/BCRP | CTCTTCGGCTTGCAACAACT | TTCTCCTCCAGACACACCAC |
Nanog | AGAAGGCCTCAGCACCTAC | GGCCTGATTGTTCCAGGATT |
Oct3/4 | CTTGAATCCCGAATGGAAAGGG | GTGTATATCCCAGGGTGATCCTC |