Fig.2

Dox-inducible Tet-on system induced SOX18 expression. A Phase-contrast micrographs of undifferentiation in Dox-inducible Sox18-iPS cells. One representative image from five independent experiments is shown. Scale bar = 300 μm. B Kusabira orange (KO) expression levels were analyzed using flow cytometry. Viable cells were gated to analyze the expression of surface markers using forward scatter versus side scatter (FSC vs. SSC). Representative data from one out of three independent experiments is shown. C Cells were treated with 0.1 ng/mL Dox, and the SOX18 expression levels was analyzed using qRT-PCR. Data are represented as the mean ± SD from three independent experiments (**p < 0.01). D Nanog and Oct3/4 expression level were analyzed using qRT-PCR. In the cells of the control group (non-transduction), the expression level of the gene was set to 1.0. Data are represented as the mean ± SD from three independent experiments