Fig. 7
From: Apicobasal transferrin receptor localization and trafficking in brain capillary endothelial cells
IF evaluation of TfR transcellular transport capability in BECs. Schematic illustration of the experimental approach for the receptor transcellular transport capability in the apical-to-basal (A-B) and basal-to-apical (B-A) direction using an immunocytochemistry assay a. Micrographs were obtained from confocal microscopy using 40 × magnification, b, with representative images showing the transcellular transport capability (green), control with secondary antibody only (Ctrl), and Hoechst staining of nuclei. Scale bar is equal to 10 μm. c Semi-quantitative analysis using spot detection, with data presented as spots/cell (5) normalized within each experimental replicate, and presented as mean ± SEM. Statistical significance was tested using t-test