Fig. 6
From: Apicobasal transferrin receptor localization and trafficking in brain capillary endothelial cells
Immunofluorescence evaluation of TfR transcellular transport capability in BECs. Representative confocal images of apical and basal internalized TfR (green) and EEA1 (red) co-localization at 10 min a, analyzed using IMARIS spot segmentation and co-localization analysis. Hoechst stain of nuclei is blue. Graph shows the semi-quantification of TfR internalized from either the apical membrane (dark grey) or basal membrane (light grey), and co-localization with the markers of the intracellular compartments b. Significance was tested using two-way ANOVA with Tukey’s multiple comparison test