Fig. 3
From: Apicobasal transferrin receptor localization and trafficking in brain capillary endothelial cells
Validation of the in vitro model polarization by directional Rhodamine 123 transport. The apical-to-basal A, B and basal-to-apical B, Atransendothelial permeability of two concentrations of the P-glycoprotein substrate Rhodamine 123 (3 and 30 µM) was assessed in the applied in vitro BBB model. The apparent permeability (Papp) for B, A transport was significantly higher than for A, B transport for both concentrations, indicating efflux pump activity and apicobasal polarity for the efflux transporter. Data are presented as mean values (± SEM). Significance was tested using t-test