Fig. 2
From: Apicobasal transferrin receptor localization and trafficking in brain capillary endothelial cells
TfR expression in brain endothelial cells. Expression of the transferrin receptor and other receptor targets in the applied in vitro model was investigated by qPCR (a) with graph showing the relative mRNA gene expression levels (2−ΔΔCt), with data normalized to the housekeeping genes β2-microglobulin, Ribosomal Protein L4, TATA-Box Binding Protein, and Hypoxanthine Phosphoribosyl- transferase 1. TfR protein level expressions were investigated by Western blotting (b) using anti-TfR and ß-actin antibody on pBEC and pMicroC lysate. Normal IF staining and confocal microscopy of pMicroC and NCC established pBECs furthermore revealed TfR expression (green), with Hoechst stain of nuclei (blue) (c). Scale bar is equal to 10 μm