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Fig. 7 | Fluids and Barriers of the CNS

Fig. 7

From: The new insight into the inflammatory response following focused ultrasound-mediated blood–brain barrier disruption

Fig. 7

Differential gene expression analysis of subtype of astrocyte in brain tissue post-FUS BBBD. (A) Heat maps depicting log2FC values for astrocyte reactivity-specific marker genes, in response to FUS-BBBD (0.25 MPa and 0.42 MPa). The DEGs were divided into pan-reactive (black), A1-specific (red), and A2-specific (green) transcripts in the RNA-seq dataset. Blue and red indicate a decrease and increase in expression compared with the sham control, respectively. (B) Gene expression correlation between qRT-PCR and RNA-seq data Serpina3n, vimentin, GFAP, and Lcn2 are pan-astrocyte-specific genes. H2-D1, Gbp2, Psmb8, and Srgn are A1-specific genes. Emp1, Tgm1, Tm4sf1, and CD14 are A2-specific genes. The blue columns represent the qRT-PCR results (left y-axis). The red lines indicate the FPKM values (right y-axis). The error bars show the standard deviation (STDEV) for the replicates in each experiment. *P < 0.05, **P < 0.01, ***P < 0.001 (two-tailed Student’s t-test; vs. Sham)

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