Fig. 5

Histological evaluation of glia cell expression in the targeted brain tissues post-FUS BBBD. (A–B) Immunohistochemical analysis of glial cell expression. The brain thalamus sections were stained with Abs against Iba-1(microglia marker, green), GFAP (astrocyte marker, red), or DAPI (blue) at 1–48 h after differential FUS parameter-mediated BBBD in 0.25 MPa (A) or 0.42 MPa (B) (n = 2). (C-D) The bar graphs represent the fluorescence intensities for Iba-1 and GFAP. The values were calculated from each of the eight ROIs per hemisphere for the BBBD region (magnification, ×20). Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 (two-tailed Student’s t-test; vs. Sham). (See Figure S5 for representative whole-brain sections stained for Iba-1 and GFAP)