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Fig. 4 | Fluids and Barriers of the CNS

Fig. 4

From: The new insight into the inflammatory response following focused ultrasound-mediated blood–brain barrier disruption

Fig. 4Fig. 4

The molecular expression associated with the inflammatory response following FUS- BBBD. (A, C) Scatterplot analysis of the inflammatory response (A) and NF-κB pathway-related GO terms (C) of DEGs in the 0.25 MPa (blue line) and 0.42 MPa (red line) groups. Scale bar indicates significant enrichment terms (adjective P < 0.05). The circle size indicates the number of DEGs corresponding to the GO terms. (B, D) Heat map of genes associated with the inflammatory response (B) and NF-κB family-related GO terms (D) at 0.25 MPa (blue line) and 0.42 MPa (red line). (E) Heap map of the 10 genes corresponding to KEGG:04064 (NF-κB signaling pathway). The bar represents the scale of the expression levels for each gene (log2FC) in the color tape from low (blue) to high (red). (F) Evaluation of the phosphorylation status of IκBa (40 kDa) and p65 (65 kDa) protein expression was analyzed by western blot analysis in rat brain tissues at 1–48 h after BBBD. GAPDH was used as a loading control. (G) Densitometric analysis of western blot results presented in (F). Western blots were normalized to total protein and densitometric analysis was performed using image processing software. Values are mean ± standard error of the mean (SEM) in two independent experiments. (H) Verification of correlation between RNA-seq data and qRT-PCR of six representative transcripts. The light blue (0.25 MPa) and dark blue (0.42 MPa) columns represent the qRT-PCR results (left y-axis). The red lines indicate the FPKM values (right y-axis). The error bars show the standard deviation (STDEV) for the replicates in each experiment. *P < 0.05, **P < 0.01, ***P < 0.001 (two-tailed Student’s t-test; vs. Sham)

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