Fig. 2

Transcriptome analysis of differentially expressed genes (DEGs) in the brain after FUS-BBBD. (A–B) Hierarchical clustering heat map with all the DEGs based on log2 FPKM values (fold-change > 2, P < 0.05; independent sample t-test, n = 2, per time point). The heat map indicates DEGs between the sham control and five samples of each time point after BBBD at 0.25 MPa (A) and 0.42 MPa (B). The red and blue colors indicate upregulation and downregulation, respectively (C-D) The number of DEGs between the sham control and each time point samples post-BBBD are shown as upregulated (red) or downregulated (blue) (fold-change > 2, P < 0.05). GO functional analysis of DEGs: 0.25 MPa (E–F) and 0.42 MPa (G-I). The distribution of GO terms of DEG was annotated in three ontology categories: biological process (blue; E, G), cellular component (yellow; H), and molecular function (green; F, I) (adjusted p-value; **P < 0.01, ***P < 0.001)