Fig. 5

hpBMEC and hiBMEC had different TCA and FAO gene and protein levels, but similar U-¹³C₆-glucose labeled metabolite fractions. A TCA cycle and FAO enzyme expression, measured by RNA sequencing. Red = higher in hiBMEC, blue = higher in hpBMEC (LogFC > 0.6). n = 3 biological replicates. B, C TCA cycle and FAO enzyme protein levels, measured by Western blot. Red = higher in hiBMEC, blue = higher in hpBMEC (significant with p < 0.05, LogFC > 0.6). Blots shown are representative of three separate experiments. n = 9 biological replicates. D Glucose labeled fraction of TCA metabolites in hpBMEC and hiBMEC incubated with U-¹³C₆-glucose for 24 h and then analyzed by LC–MS. n = 6 biological replicates. *p < 0.05 and LogFC > 0.6 using a Mann–Whitney test