Fig. 4

hpBMEC and hiBMEC showed differences in glycolytic enzyme gene and protein levels, but similar U-¹³C₆-glucose labeled metabolite fractions. A Glucose transporter, glycolysis, and glycolysis side branch pathway enzyme gene expression, measured by RNA sequencing. Red = higher in hiBMEC, blue = higher in hpBMEC (LogFC > 0.6). n = 3 biological replicates. B, C Western blots with quantification of glucose transporter and glycolytic enzyme protein levels from hpBMEC and hiBMEC. Red = higher in hiBMEC, blue = higher in hpBMEC (significant with p < 0.05, LogFC > 0.6). Blots shown are representative of three separate experiments. n = 9 biological replicates. D, E Glucose labeled fraction of glycolytic (D) and glycolytic side branch pathway (E) metabolites in hpBMEC and hiBMEC incubated with U-¹³C₆-glucose for 24 h and then analyzed by LC–MS. n = 6 biological replicates. *p < 0.05 and LogFC > 0.6 using a Mann–Whitney test