Fig. 2

hpBMEC had higher glycolytic and oxidative glucose metabolism than hiBMEC, but similar proportions of glycolytic glucose metabolism. A Representative confocal images of GLUT1, ZO-1, occludin (all red), and hoechst (nuclei, blue) in hpBMEC and hiBMEC. Scale = 100 µm. B TEER of hpBMEC and hiBMEC measured on confluent monolayers on 0.4 µm pore Transwell filters. C Glucose uptake, lactate production, and lactate:glucose ratio in hpBMEC and hiBMEC cultured in 5.5 mM glucose over 24 h, as measured by YSI. D GlycoPER (glycolysis) and E OCR (oxidative respiration) in hpBMEC vs. hiBMEC measured via Seahorse assays, normalized to cell count. n = 6 biological replicates; *p < 0.05 using a Mann–Whitney