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Fig. 4 | Fluids and Barriers of the CNS

Fig. 4

From: Novel localization of folate transport systems in the murine central nervous system

Fig. 4

Relative expression of the folate transport systems in various models of the brain barriers. A Relative mRNA expression of mouse Slc19a1 (RFC), Slc46a1 (PCFT), and Folr1 (FRα) was assessed in isolated mouse CP tissue (Mouse CP), primary cultures of mouse brain microvascular endothelial cells (Mouse BBB) and in immortalized mouse AB cells (Mouse AB). Results are presented as mean relative mRNA expression normalized to the housekeeping gene mouse cyclophilin B (n = 4). B–D Representative immunoblots from three separate experiments demonstrating protein expression of RFC, PCFT and FRα in isolated mouse CP tissue, in primary mouse brain microvascular endothelial cells, and in immortalized mouse AB cells. HEK293 and HepG2 cells were used as positive controls for the three transport systems, with mouse kidney used as an additional positive control for FRα. Multiple protein bands for RFC and PCFT represent differential glycosylation

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