Fig. 5
From: Transcriptomic analysis of a 3D blood–brain barrier model exposed to disturbed fluid flow
A Axial cross-section of the vessel showing the location of GFP-labeled endothelial cells, and smooth muscle and astrocytes stained with anti-alpha-SMA and anti-GFAP, respectively. Scale = 100 μm. B Radial cross-sectional images of the vessel lumens indicating differences in lumican expression between scrambled (i) and knockdown (ii) conditions. Scale bar = 100 μm. C ZO-1 localization to cell–cell junctions of knockdown cells with lumican added exogenously into the matrix in the fully developed (C,i) compared to the disturbed (C,ii) region. Scale bar = 25 μm. D Permeability quantification compared to static controls in fully developed and disturbed regions, * p < 0.05. n = 3 per condition