Fig. 3
From: Transcriptomic analysis of a 3D blood–brain barrier model exposed to disturbed fluid flow
A qRT-PCR quantification of differentially expressed genes identified by RNA sequencing. B Lumican quantification between portions of the vessel exposed to steady and fully developed. C-D Portions of fully developed (C) and disturbed (D) regions from the 3D vessels stained with anti-ZO-1 (red) and anti-lumican (green) and DAPI (blue). Scale bar = 25 μm. E Protein quantification of lumican expression from monolayers exposed to uniform shear stress (“fully developed”) or a shear gradient (“disturbed”). * p < 0.05