Fig. 4

Vessel diameter change and RBC velocity are affected in diabetic APP/PS1 mice. A When we compared vessels with CAA, we observed a significant reduction in vessel diameter change in prediabetic APP/PS1 animals and larger reductions were observed in APP/PS1xdb/db mice [F_(3,1172) = 29.87, **p < 0.01 vs. rest of the groups, ‡‡p < 0.0.1 vs. APP/PS1-RD and APP/PS1]. Data are representative of 4–6 mice (APP/PS1 n = 6, APP/PS1xdb/db n = 4, APPP/PS1-RD n = 5, APP/PS1-HFD n = 5) (mean ± SEM). B A reduction of RBC velocity was observed in APP/PS1xdb/db mice that reached statistical significance when 25–50 µm in diameter vessels were analyzed. Whereas a similar profile was observed in prediabetic mice, differences did not reach statistical significance (< 25 µm [F_(3,160) = 0.589, p = 0.623]; 25–50 µm [F_(3,123) = 4.227, ‡‡p = 0.007 vs. APP/PS1-RD and APP/PS1]). Individual RBC are represented from 5–15 line scans (APP/PS1 < 25 µm n = 15, 25–50 µm n = 11, APP/PS1xdb/db < 25 µm n = 15, 25–50 µm n = 8, APP/PS1-RD < 25 µm n = 9, 25–50 µm n = 6, APP/PS1-HFD < 25 µm n = 5, 25–50 µm n = 9). C) Representative examples of RBC line scans in CAA vessels from APP/PS1-RD, APP/PS1-HFD, APP/PS1 and APP/PS1xdb/db mice