Table 1 Primary antibodies used for immunofluorescence

CD31

1:200

Rb, polyclonal, ref. ab32457

Abcam

VE-Cadherin^a

1:25

Ms, clone BV9

Santa Cruz

ZO-1^b

1:100

Ms, clone ZO1-1A12

Invitrogen

Occludin^a

1:200

Ms, clone OC-3F10

Invitrogen

Claudin-5^a

1:50

Ms, clone 4C3C2

Invitrogen

Vimentin

1:100

Ms, clone V9

Invitrogen

Desmoplakin I/II

1:50

Ms, clone A-1

Santa Cruz

EMA (Mucin 1)

1:25

Ms, clone VU4H5

Santa Cruz

E-Cadherin

1:100

Ms, clone G-10

Santa Cruz

Laminin

1:50

Rb, polyclonal, ref. PA1-16730

Invitrogen