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Fig. 2 | Fluids and Barriers of the CNS

Fig. 2

From: Development of a multicellular in vitro model of the meningeal blood-CSF barrier to study Neisseria meningitidis infection

Fig. 2

Characterization of the iBEC-LMC co-culture model. a Transmission electron microscopy (TEM) of iBECs and LMCs co-cultured on transwell for 2 days. A widefield image of a semithin cross-section of the embedded model (middle, top) is presented in addition to electron micrographs of ultrathin sections. Labeled structures: cell junctions (J), Golgi apparatus (G), rough endoplasmic reticulum (ER), mitochondria (M), vesicles (V), lamellar bodies (LB). b Immunofluorescence staining of iBEC (top) and LMC (bottom) monolayers on either side of transwell membranes for endothelial adherens junction proteins (CD31, VE-Cadherin), tight junction components (claudin-5, ZO-1, occludin, E-cadherin), and meningioma markers (vimentin, desmoplakin I/II, epithelial membrane antigen), performed after 2 days of co-culture. Nucleus staining with DAPI (blue). Scale bars represent 20 µm. c Transendothelial electrical resistance (TEER) of iBEC monoculture (black), direct (blue) and indirect (green) iBEC-LMC co-culture over a time-course of 14 days. d Sodium fluorescein permeability (NaF Pe) of iBEC mono and co-culture models on day 2 of co-culture, determined according to previously describe protocols [48]. All data presented as mean ± SD from three independent experiments and iBEC differentiations performed in triplicate (n = 9). *p < 0.05, **p < 0.01, ***p < 0.001, **** p < 0.0001; ANOVA followed by Dunnett’s multiple comparisons test; direct (blue) and indirect (green) co-culture vs monoculture

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