Fig. 1From: Development of a multicellular in vitro model of the meningeal blood-CSF barrier to study Neisseria meningitidis infectionSchematic representation of iPSC-derived brain endothelial-like cell (iBEC) differentiation and co-culture with leptomeningeal cells (LMCs). iBEC differentiation as previously described [44,45,46, 48]. On day 8, LMC are seeded on the underside of transwells or on standard tissue culture plates. After 4Â h of incubation, iBECs are purified onto the apical side of the transwells. Co-cultures were cultivated in EC medium with Pen/Strep for 1Â day, before removal of factors added to the basal EC medium. Experiments were conducted on day 10 of differentiation (day 2 of co-culture). Figure created with BioRender.comBack to article page