Fig. 6
From: The choroid plexus: a door between the blood and the brain for tissue-type plasminogen activator
The Finger domain of tPA allows the transport of tPA and BSA across the BCSF barrier in vivo. A Experimental design: mice were injected intravenously with 50 µg of tPA555/ ΔF-tPA488 or F-BSA555/BSA488, CSF and brains were harvested 30 or 60 min later for protein analysis by electrophoresis and immunohistochemistry, respectively. B–C, Representative confocal photomicrographs and corresponding quantification (mean ratio of CPECs containing tPA555 or ΔF-tPA488 over total CPECs ± SEM, n = 5) of CPs sections with tPA555 (magenta), ΔF-tPA488 (green) and TTR (white) staining; scale bar 40 µm and 20 µm in the magnified inserts. * significant difference (p < 0.05), $ significant difference from 0 min (p = 0.0022). D–E Representative confocal photomicrographs and corresponding quantification (mean ratio of CPECs containing tPA555 or ΔF-tPA488 over total CPECs ± SEM, n = 5) of CPs sections with F-BSA555 (magenta), BSA488 (green) and TTR (white) staining; scale bar 40 µm and 20 µm in the magnified inserts. * significant difference (p < 0.05), $ significant difference from 0 min (p = 0.0079). F–G, Representative electrophoresis and corresponding quantification for tPA555 and F-BSA555 levels in CSF samples at 30 and 60 min (mean ± SEM, n = 4)