Fig. 5
From: The choroid plexus: a door between the blood and the brain for tissue-type plasminogen activator
The Finger domain of tPA allows the internalization of tPA and BSA by CPECs ex vivo. A Schematic representation of the functional domains of tPA and its mutant form ΔF-tPA, deleted of the finger domain. B Electrophoresis of 5 µg of tPA555 (magenta) and ΔF tPA488 (green) in SDS-PAGE and revelation in fluorescence. C–D Representative photomicrographs and corresponding quantifications of CPs explants exposed to tPA555 (40 nM) and ΔF-tPA488 (40 nM, 200 nM or 400 nM) for 15 min (n = 4, mean ± SEM). E Schematic representation of BSA and its mutant form F-BSA, adding the finger domain of tPA. F Electrophoresis of 5 µg of BSA488 (green) and F-BSA555 (magenta) in SDS-PAGE and revelation in fluorescence. G, H Representative photomicrographs and corresponding quantifications of CPs explants exposed to BSA488 (40 nM) and F-BSA555 (40 nM) for 15 min (n = 4, mean ± SEM). TTR staining (white) is shown on the merged images (C and F). Scale bar: 40 µm