Fig. 3

CPECs uptake tPA in vivo. A Experimental design: mice were injected intravenously with 50 µg of tPA⁵⁵⁵ and BSA⁴⁸⁸, CSF and brains were harvested 10, 30, 60 or 120 min later for protein analysis by electrophoresis and immunohistochemistry, respectively. B Confocal photomicrographs of CPs sections (representative of n = 5) with tPA⁵⁵⁵ (magenta), BSA⁴⁸⁸ (green) and TTR (white) staining, scale bar 40 µm and 20 µm in the magnified inserts. C Confocal photomicrograph of a CP section (representative of n = 5), tPA⁵⁵⁵ (magenta) and TTR (white) staining, panels show projection over the XZ axis (bottom) and over the YZ axis (right), scale bar 10 µm. D Graph shows mean ratio ± SEM (n = 5) of CPECs containing tPA⁵⁵⁵ or BSA⁴⁸⁸ over total CPECs. The magenta curve corresponds to tPA⁵⁵⁵ and the green one to BSA⁴⁸⁸, * significant difference (p < 0.05), $ significant difference from 0 and 120 min (p = 0.008). E Western-Blot for tPA levels in the CSF samples at each time-point. F Confocal photomicrographs acquired in the brain parenchyma (representative of n = 5) with tPA⁵⁵⁵ (magenta) and NeuN (white) staining, scale bar 40 µm and 10 µm in the magnified inserts