Fig. 2

Secondary hit validation for nuclear receptor ligands that regulate leptin transport. For outcomes from the primary screen, refer to Additional file 1: Table S4. A Compiled datasets represent the basolateral chamber leptin concentration corresponding to the noted ligand treatment, normalized to the DMSO control. Each data point is the mean of a single biological replicate calculated using three Transwell filters (n = 3 technical triplicates). Data are represented as mean ± standard deviation from n = 3 biological replicates per nuclear receptor ligand. A one-way ANOVA with a Tukey post hoc test was used to evaluate the statistical significance across conditions (*p < 0.05). B Representative TEER measurements before and after the leptin transport assay. Data are represented as mean ± standard deviation from 3 Transwell filters (n = 3 measurements per filter) per condition used in a single experiment. Trends were confirmed across two additional biological replicates, where no significant differences in TEER were noted as a function of the assayed ligand