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Fig. 1 | Fluids and Barriers of the CNS

Fig. 1

From: Nuclear receptor ligand screening in an iPSC-derived in vitro blood–brain barrier model identifies new contributors to leptin transport

Fig. 1

Establishment of leptin transport assay. A Experimental timeline for iPSC differentiation and initiation of assays. Leptin transport was always assessed on Day 1 of subculture. Modifications were made on Day 0 or/and 1 of subculture to reflect different experimental setups (time points indicated by asterisks). B Temporal leptin transport through BMEC-like cells as a function of leptin concentration in the apical chamber. Mass of leptin in the basolateral chamber was measured using an ELISA and a standard curve. Percent of leptin transported was calculated by dividing mass in the basolateral chamber by mass in the apical chamber. Data are presented as mean ± standard deviation from n = 3 biological replicates. C BMEC-like cells demonstrate the high-fidelity passive barrier function before and after the leptin transport assay. TEERs are represented as mean ± standard deviation in a single biological replicate (n = 3 measurements from each filter). Trends were confirmed in two additional biological replicates

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