Fig. 4
From: VHHs as tools for therapeutic protein delivery to the central nervous system
Characterization of anti-hTfR Nb188 in TfR binding and shuttling over the BBB. A Binding kinetics of Nb62 and Nb188 to immobilized mTfR and hTfR, respectively, were determined by BLI using the Octet system. B TfR hAPI KI mice body temperature measurements after 250 nmol/kg intravenous injections of the indicated VHH fused to NT(8-13). Bar graphs represent mean ± SEM (n = 3 per group). Statistical test: two-way ANOVA with repeated measures and Sidak’s multiple comparisons test. (significant time*treatment interaction effect ****p < 0.0001)