Fig. 4
From: ROS attenuates TET2-dependent ZO-1 epigenetic expression in cerebral vascular endothelial cells
5hmC is markedly reduced in endothelial cells of aged human brains and it is positively correlated with the expression of ZO-1. A IHC staining of 5hmC in endothelial cells of adult and aged brains. Insets show the higher magnification of selected regions. B Semi-quantitative analysis of 5hmC positive staining in endothelial cells of adult and aged brains. Scale bars: 50 μm. Data were shown as the mean ± SEM. The p values were determined by the two-tailed t-test. Values of p < 0.05 were considered statistically significant. * denoted p = 0.0459. C IHC staining of ZO-1 in endothelial cells of adult and aged brains. Insets show the higher magnification of selected regions Scale bars: 50 μm. D Semi-quantitative analysis of 5hmC positive staining in endothelial cells of adult and aged brains. Scale bars: 50 μm. Data were shown as the mean ± SEM. The p values were determined by the two-tailed t-test. Values of p < 0.05 were considered statistically significant. * denoted p = 0.0375. E The correlation between 5hmC IRS and ZO-1 IRS in endothelial cells of human brains (Pearson correlation γ = 0.5518, **p = 0.001)