Fig. 3
From: ROS attenuates TET2-dependent ZO-1 epigenetic expression in cerebral vascular endothelial cells
TET2 silencing selectively inhibited the expression of ZO-1 and increased endothelial permeability. A The expression of ZO-1 in endothelial cells was decreased after Tet2 was knocked down by siRNA. B hMeChip-PCR detected the 5hmC modification of CpG dinucleotides of the promoter region of ZO-1 in endothelial cell before and after knocking down TET. C Quantitative data analysis of the permeability of the endothelial monolayer with the assistance of FITC-dextran. D, E Representative images of FITC-dextran extravasation in the mouse brain. In the brain of wild-type mice, very weak fluorescence appeared in the brain parenchyma (D). In the brain of Tet2 KO mice, the brain parenchyma was filled with bright fluorescent FITC-dextran (10 kDa) on a bright green background indicative of extravasation of FITC-dextran across the BBB (E). Scale bars: 100 μm. All data were shown as the mean ± SEM. The p values were determined by the two-tailed t-test. Values of p < 0.05 were considered statistically significant. *and ** denoted p < 0.05 and p < 0.01, respectively