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Fig. 2 | Fluids and Barriers of the CNS

Fig. 2

From: ROS attenuates TET2-dependent ZO-1 epigenetic expression in cerebral vascular endothelial cells

Fig. 2

ROS epigenetically compromised ZO-1 expression. A Bend.3 and HUVECs were treated with or without 10 μM H2O2 for 6 h and supplemented with or without 1 mM NAC for an extended 6 h for blotting 5hmC as indicated. B The expression of ZO-1 in Bend.3 and HUVECs treated with or without 10 μM H2O2 for 6 h and supplemented with or without 1 mM NAC for an extended 6 h. C Semi-quantitative analysis of ZO-1 positive bands of endothelial cells. D Immunofluorescence staining of ZO-1 expression in endothelial cells treated with H2O2 and supplemented with NAC as indicated. E HUVECs and HCMECs treated with H2O2 then supplement with NAC. Scale bars: 50 μm. All data were shown as the mean ± SEM. The p values were determined by the two-tailed t-test. Values of p < 0.05 were considered statistically significant. *and ** denoted p < 0.05 and p < 0.01, respectively

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