Fig. 2
From: The effect of A1 and A2 reactive astrocyte expression on hydrocephalus shunt failure
Expression of C3, EMP1 astrocyte activation genes assessed by RNAscope fluorescent in situ hybridization on obstructed and non-obstructed shunts. A SLC1A3 was used as an astrocyte marker to confirm that C3 and EMP1 signal represented the A1 and A2 astrocytes specifically. Data for obstructed and non-obstructed shunts are shown. For normalization, the C3 and EMP1 signals were dividing by SLC1A3 signals. Statistical analysis was carried out using two-way ANOVA; *p < 0.05. B Representative RNAscope fluorescent in situ hybridization images for obstructed and non-obstructed astrocyte gene C3 (red) and EMP1(yellow) showing colocalization with the astrocyte marker SLC1A3 (green). Separated channels for C3 and EMP1 for each condition are also presented (scale bar = 500 μm)