From: Microglia-derived CCL2 has a prime role in neocortex neuroinflammation
Primary Abs | Host IgG | Concentration (µg/µl) | Supplier | Catalogue No |
---|---|---|---|---|
CCL2/MCP1 | Mo IgG | 15.5 | Novus biological | NBP2-22,115 |
CD31 | Rat IgG | 0.6 | Abcam | Ab7388 |
CD45 | Rat IgG | 5 | Novus biological | NB110-93,609 |
Claudin-5 | Mo IgG1 | 16.6 | Thermo fisher | 18–7364 4C3C2 |
Collagen type IV | Rb IgG | 5.0 | Acris | R1041 |
GFAP | Rat IgG | 6.7 | Invitrogen | 13–0300 |
GFAP | Rb IgG | 10 | Immunostar | 22,522 |
GFP | Rb IgG | 5.0 | Molecular probes | A6455 |
IBA1 | Gt IgG | 5 | Abcam | ab5076 |
NG2 | Rb IgG | 5.5 | Millipore | AB5320 |
Occludin | Rb IgG | 5.0 | Thermo fisher | 71–1500 |
SALL1 | Rb IgG | 5 | Abcam | Ab31526 |
TMEM119 | Mo IgG | 10 | Abcam | ab209064 |
Secondary Abs and reagents | Concentration (µg/µl) | Supplier | Catalogue No | |
---|---|---|---|---|
Biotinylated horse anti-mouse IgG | 3.75 | Vector | BA-2000 | |
Biotinylated goat anti-rabbit IgG | 3.75 | Vector | BA-1000 | |
Biotinylated goat anti-rat IgG | 3.75 | Vector | BA-9400 | |
Biotinylated horse anti-goat IgG | 3.75 | Vector | BA-9500 | |
Streptavidin-Alexa 488 | 6.6 | Invitrogen | S-11223 | |
Streptavidin-Alexa 555 | 6.6 | Invitrogen | S-21381 | |
Goat anti-rabbit Alexa 568 | 6.6 | Invitrogen | A11011 | |
Goat anti-rabbit Alexa 488 | 6.6 | Invitrogen | A11070 | |
Goat anti-rat Alexa 555 | 2.5 | Invitrogen | A21434 |