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Table 1 List of primary and secondary antibodies applied to IHC and RNAscope IHC/ISH

From: Microglia-derived CCL2 has a prime role in neocortex neuroinflammation

Primary Abs

Host IgG

Concentration

(µg/µl)

Supplier

Catalogue No

CCL2/MCP1

Mo IgG

15.5

Novus biological

NBP2-22,115

CD31

Rat IgG

0.6

Abcam

Ab7388

CD45

Rat IgG

5

Novus biological

NB110-93,609

Claudin-5

Mo IgG1

16.6

Thermo fisher

18–7364 4C3C2

Collagen type IV

Rb IgG

5.0

Acris

R1041

GFAP

Rat IgG

6.7

Invitrogen

13–0300

GFAP

Rb IgG

10

Immunostar

22,522

GFP

Rb IgG

5.0

Molecular probes

A6455

IBA1

Gt IgG

5

Abcam

ab5076

NG2

Rb IgG

5.5

Millipore

AB5320

Occludin

Rb IgG

5.0

Thermo fisher

71–1500

SALL1

Rb IgG

5

Abcam

Ab31526

TMEM119

Mo IgG

10

Abcam

ab209064

Secondary Abs and reagents

Concentration (µg/µl)

Supplier

Catalogue No

Biotinylated horse anti-mouse IgG

3.75

Vector

BA-2000

Biotinylated goat anti-rabbit IgG

3.75

Vector

BA-1000

Biotinylated goat anti-rat IgG

3.75

Vector

BA-9400

Biotinylated horse anti-goat IgG

3.75

Vector

BA-9500

Streptavidin-Alexa 488

6.6

Invitrogen

S-11223

Streptavidin-Alexa 555

6.6

Invitrogen

S-21381

Goat anti-rabbit Alexa 568

6.6

Invitrogen

A11011

Goat anti-rabbit Alexa 488

6.6

Invitrogen

A11070

Goat anti-rat Alexa 555

2.5

Invitrogen

A21434