Fig. 2
From: Microglia-derived CCL2 has a prime role in neocortex neuroinflammation
Representative images of neocortex sections from naïve (a), EAE-affected (b; cs 2.0) and EAE-affected MSC-treated (c; cs 1.5) mice, sacrificed 24 h after MSC treatment, double immunostained for IBA1 and CCL2. a Microglia-like cells are faintly stained by IBA1 (arrows) and a CCL2-stained microvessel is recognizable (V). b The two markers are extensively colocalized on microglia-like cells in the EAE neocortex, defining hypertrophic cells characterized by ‘thorny’ processes and a bushy aspect (arrows); IBA1 prevails on the cell body and is fully colocalized with CCL2 on cell processes; note aspects of cell fusion (asterisk). c In the cortex of EAE-affected MSC-treated mice, IBA1/CCL2-stained microglia-like cells show a simplified morphology and smaller size but the same distribution of the two markers (arrows). TOPRO-3 nuclear counterstaining. Scale bars: a–c 25 μm