Fig. 11

Representative images of neocortex sections from EAE-affected (a, c, e; cs 2.5,3.5, 2.5, respectively) and EAE-affected MSC-treated (b, d, e; cs 2.0, 2.25, 2.25, respectively) mice, sacrificed at 6 h (6 h), 24 h (24 h), and 10 days (10d) after MSC treatment, and immunostained for occludin. a, c, e In EAE-affected mice, the occludin junctional staining pattern is characterized by rows of fine puncta at 6 h and persists at 24 h and 10d (arrows). b, d, f Upon treatment with MSCs, the occludin pattern continuity is undisturbed (arrows). TOPRO-3 nuclear counterstaining. Scale bars: a–f 25 µm