Fig. 10
From: Microglia-derived CCL2 has a prime role in neocortex neuroinflammation
Representative images of claudin-5 immunostained neocortex sections from EAE-affected (a, c, e; cs 2.5, 3.5, 2.5, respectively) and EAE-affected MSC-treated (b, d, e; cs 2.0, 2.25, 2.25, respectively) mice, sacrificed at 6 h (6 h), 24 h (24 h), and 10 days (10d) after MSC treatment. a, c, e In EAE, the claudin-5 junctional staining pattern is lost at the shorter examined interval from the disease onset (a, arrows), as well as at the subsequent examined intervals (c, e; arrows). b, d, f A linear and continuous staining pattern for claudin-5 is recognizable on cortex microvessels of MSC-treated mice (arrows). TOPRO-3 nuclear counterstaining. Scale bars: a-f 25 µm