Fig. 4

Pathophysiology of the ICH model and decreased Notch1 signaling in ICH model mice. A Cylinder test performed 24 h after collagenase injection (n = 10 mice/group). B Representative brain sections showing hematoma volume in the collagenase-induced ICH mouse model. Scale bar: 10 mm. C Representative coronal brain sections showing IgG staining, used to evaluate BBB disruption. Scale bar: 100 μm. D Intensity of IgG staining, quantified using ImageJ (n = 3 mice, 7 slides per group). Decreased Notch1 (E) and Hes1 (F) protein levels in ICH model mice (n = 6 for Vehicle, n = 10 mice for ICH group). G Mitochondrial respiration in the striatum of Vehicle and ICH groups by OCR analysis (n = 6 mice/group). H OCR analysis values. State 2: basal respiration; State 3: after ADP injection; State 4o: after oligomycin injection; State 3u: after CCCP injection. I, J Total OxPhos complex protein levels. Data are presented as means ± SD from three independent experiments performed under the same conditions (*P < 0.05, **P < 0.01, ***P < 0.001 vs. Vehicle)