Fig. 1

Mitochondrial dysfunction in isolated brain microvessels from TEKCRIF1 KO mice. A Schematic depiction of methods for isolating microvessels from the brain. B Morphology of isolated brain microvessels from a TEKCRIF1 WT mouse. Scale bar: 100 μm. C, D Western blot analysis showing the decrease in Crif1 protein in KO mice (n = 7 mice/group). E, F Immunofluorescence staining showing the decrease in Crif1, NDUFA9 (mitochondrial complex I subunit), and CD31 (EC marker) in isolated microvessels from TEKCRIF1 KO mice (n = 5 mice/group 6 slides per group). Scale bar: 20 μm. Immunofluorescence staining intensity was quantified using ImageJ. Data are presented as means ± SD from three independent experiments performed under the same conditions (**P < 0.01, ***P < 0.001 vs. WT)