From: Extracellular vesicles through the blood–brain barrier: a review
Mechanism | Author | Cells and EVs | Inhibitor (concentration) | Pre-treatment duration | Incubation with EVs | Uptake of Evs |
---|---|---|---|---|---|---|
Macropinocytosis | Morad et al | BMECs + TdTom-Br-EVs | EIPA (100 μM); cytochalasin D (500 nM) | 30 min | 3 h | Decreased |
Chen et al | BMECs + PKH26-labeled exosomes | EIPA (1 mM); cytochalasin D (20 μM) | 30 min | 1 h | Decreased | |
Clathrin-dependent endocytosis | Morad et al | BMECs + TdTom-Br-EVs | Chlorpromazine (20 μM); ML141 (100 μM) | 30 min | 3 h | Decreased |
Chen et al | BMECs + PKH26-labeled exosomes | Chlorpromazine (15 μM) | 30 min | 1 h | Decreased | |
Lipid raft/caveolae-dependent endocytosis | Morad et al | BMECs + TdTom-Br-EVs | Filipin III (10 μM) | 30 min | 3 h | No effect |
Chen et al | BMECs + PKH26-labeled exosomes | Filipin III (5 μM); MβCD (5 mM); nystatin (5 μM) | 30 min | 1 h | Decreased |