Table 2 Characterization of extracellular vesicles

Morad et al

MDA-MB-231

Ultracentrifugation at 100000 g for 90 min at 4 °C

154.1 ± 7.0 and

158.5 ± 6.0

N.A

CD9, CD63, Alix

GM130

1 × 10¹¹ particles/mL

N.A

Tominaga et al

MDA-MB-231-luc-D3H1 and MDA-MB-231-luc-D3H2LN

Ultracentrifugation at 110,000 g for 70 min at 4 °C

100

N.A

CD63, CD9

Cytochrome C

1.2 × 10⁹ particles/mL

N.A

Chen et al

HEK 293 T cells

Ultracentrifugation 120,000 g for 2.5 h at 4 °C

96.3 ± 5.4 and 80.3 ± 2.0

N.A

CD63, CD9, CD81

N/A

3 × 10⁸ and 6 × 10⁸ particles/mL

N.A

Matsumoto et al

Human erythrocytes

SEC

205.22 ± 1.79

N.A

Alix

CD235a (RBC)

1.76 × 10⁹ particles/mL

0.68 ± 0.11 mg/dish

Kuroda et al

SK-Mel-28

ExoQuick-TC (polymer based extraction by precipitation) and MagCapture (Affinity method for phosphatidylserine)

217.0 ± 4.5

N.A

CD9,CD81,TSG101,Alix,flotillin-1

calnexin, GRP78

9 × 10⁹ particles/mL

N.A