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Table 1 Transwell models used to evaluate crossing of EVs through the BBB

From: Extracellular vesicles through the blood–brain barrier: a review

Author

Type of cells

Number of layers at crossing

Time grown

Coated with

Pore size

Additional internvention

Chen et al

Human BMECs

Monolayer

48 h

Collagen type 1

0.45 µm

N.A

Morad et al

Human BMECs

Monolayer

48 h

Fibronectin

0.40 µm

8-CPT-cAMP and RO 20–1724

Matsumoto et al

CD1 mice BMECs

Monolayer

N.A

Fibronectin and collagen type IV

0.40 µm

Hydrocortisone

Tominaga et al

Monkey BMECs and Wistar rat pericytes

Bilayer

N.A

N.A

3.00 µm

Hydrocortisone

  1. BMECs brain microvascular endothelial cells, N.A. information not available.