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Fig. 6 | Fluids and Barriers of the CNS

Fig. 6

From: Membrane transporters control cerebrospinal fluid formation independently of conventional osmosis to modulate intracranial pressure

Fig. 6

NKCC1-mediated fluid flow and contribution to ICP. a Calcein fluorescence in rat choroid plexus as a function of time with exposure to control aCSF or + 100 mOsm aCSF adjusted with mannitol, NaCl, or KCl in the absence or presence of bumetanide, when indicated by grey box, n = 5. Summarized data illustrated in right panel with statistical significance tested with one-way ANOVA followed by Tukey’s multiple comparison test, **P < 0.01, ***P < 0.001, NS: not significant. b ICP recordings during intraventricular infusion of aCSF at rates of 0.5 (n = 6) or 1.5 μl min−1 (n = 4) over 20 min. Unpaired two-tailed t-test was applied to evaluate statistically significant differences at the end of the procedure. c The intracranial pressure of rats treated with slow intraventricular infusion (0.5 μl min−1) ± bumetanide for 100 min. Controls are from b, n = 6. Data are presented as % of control ICP as a function of time with each dot representing the mean value over a 5 min period. The endpoints are demonstrated in the right panel with unpaired two-tailed t-test employed for statistical analysis, **P < 0.01

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