Fig. 9

CYM-5442 protects against BBB disruption after TBI by reducing vesicle transcytosis. A, B, C The protein levels of albumin (B) and caveolin-1 (C) as measured by WB. n = 4/group. D, E Representative immunofluorescence images of CAV-1 protein expression. n = 4/group. Bar = 100 μm. F, G TEM images of transcytosis vesicles (black arrows) in ECs (ECs) after TBI. Scale bar = 500 nm (F). Quantification of the transcytosis vesicular density (G). n = 4/group. H, I HRP-filled vesicles (white arrowheads) (H) were observed in ECs and quantified (I). n = 4/group. Scale bar = 200 nm. J Quantitative analyses of the degree of EB dye extravasation in the ipsilateral cortices of each group on day 3 after TBI. n = 6/group. K Brain water content in the whole cerebrum in each group on day 3 after TBI. n = 10/group. All data was analyzed by one-way ANOVA and Tukey’s multiple comparisons test, all values are presented as the mean ± SD. (-): TBI only; (Ctr): control shRNA; (Vec): vehicle; (OE): overexpression of Mfsd2a